Title: "Ribonomics to decipher RNA-based regulation in Arabidopsis thaliana"
Please email IIGBadmin@ucr.edu for zoom link.
Host: Xuemei Chen
Abstract: RNA-based regulation emerges as a key factor shaping the transcriptome. We are studying the function of the circadian clock-regulated Arabidopsis thaliana glycine-rich RNA-binding protein7 (AtGRP7) involved in circadian timekeeping, flowering time control, and pathogen defence. To determine the in vivo target transcripts and binding sites genome-wide we have for the first time adapted Individual nucleotide resolution crosslinking and immunoprecipitation (iCLIP) to plants. Transgenic plants expressing AtGRP7-GFP were subjected to UV crosslinking to preserve RNA-protein interactions. Upon immunoprecipitation of AtGRP7-GFP from plant lysates with GFP Trap beads®, bound RNAs were identified by Next generation sequencing and binding sites were determined at nucleotide resolution. I will discuss insights into mechanisms how AtGRP7 affects its targets, Furthermore, I will report on a role of AtGRP7 in regulating the biogenesis of selected microRNAs. Finally, I will present experiments aimed at assembling a compendium of binding sites for a suite of regulatory RNA-binding proteins. Overall, our data provide insights into posttranscriptional regulation in Arabidopsis.